By Lieshout M. N.
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X 2 m length). The temperature settings used were as follows: column at 180oC, injector and detector at 210oC. This equipment used hydrogen gas (30 ml·min-1) as a burning gas and required an air flow of approximately ten times the hydrogen gas flow (300 ml·min-1) for complete combustion. Nitrogen served as the carrier gas at a flow rate of 30 ml·min-1. 2 Materials and Methods 33 column was filled with chromosorb C101 (80-100 mesh; Sigma, Deisenhofen) and equilibrated (activated) with phosphoric acid.
23: The maximum growth rate (v max) v max S Km and Km are both constants in a enzyme-substrate-system and the reaction is proportional to the concentration of the substrate. The reaction follows a first order kinetics. 01 Km). The Michaelis-Menten equation represents a continuum for defining enzyme-catalyzed reaction. For example an experiment was started with a large amount of substrate and the kinetics of the reaction was followed over time. When there was no new substrate addition, the reaction followed zero order kinetics initially.
7: 2CH3•CO•COOH + 2H2O ' 2CH3•COOH + 2CO2 + 4